MS3 searches publicly available mass spectral libraries using the NIST MS Search Similarity method. It covers Mass Bank of North America, LipidBlast, GNPS and several other specialized mass spectral libraries.
1) Organize raw data files by chromatography types ("RP" and "HILIC") and ESI modes ("ESIPOS" and "ESINEG").
2) Convert raw data to mzML format using the MSConvert (proteowizard) software. Select the MS level "1-2".
3) Create a folder "IDSL" in your Google Drive, and create a sub-directory "LCMS" in it.
4) Create a folder for the study in "LCMS" folder, and upload the mzML files to corresponding sub-folders from #1.
5) Change the sharing setting for the "LCMS" folder to "anyone with the link can access". Copy the link.
6) Submit the link to below Google Form folder. You will receive an email with the processed results.